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Anti-human Immunoglobulins (IgG, IgA, IgM) goat IgG fraction, fluorescein-conjugated, 2 mL
Key Features and Details
Lyophilized fluorescein-5-isothiocyanate (FITC “Isomer I”)-conjugated goat IgG fraction to human immunoglobulins (IgG,IgA,IgM) and buffer salts.
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PRODUCT DESCRIPTION
Product is the lyophilized powder of fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat IgG fraction to human immunoglobulins (IgG,IgA,IgM) and buffer salts.
Application Notes
This product is suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies are recommended to avoid non-specific binding from the inherent antibodies of host animals.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Immunoassays | Immunohistochemistry (Paraffin; Acetone-Fixed; 4% PFA Frozen) | Immunofluorescence assays (IFA) | Flow Cytometry (FACS)
SPECIFICATIONS
SKU: 0855156
Base Catalog Number: 55156
Antibody Type
: Polyclonal Antibody
Concentration
: Total Protein: 16.2 mg/mL F/P Ratio: 3.8 mol/mol
Conjugate
: FITC
Formulation Details
: Lyophilized
Host
: Goat
Preparation Method
: Antibody and FITC are conjugated under defined conditions to obtain the specified molar ratio (3-6mol/mol). Conjugated protein is purified by gel filtration. The product is dialyzed into 0.02M sodiumphosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 µm filter, vialed, and lyophilized.
Purity
: Purified
Solubility
: Reconstitution: Reconstitute product with 2 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material.
Species Reactivity
: Human (IgG, IgA, IgM)
Specificity
: This product shows reactivity to human immunoglobulins (IgG;IgA;IgM); cross-reactivity to other species may exist. Antibody activity to non-immunoglobulin serum proteins is not present.
Typical Working Concentration
: Dilution Recommendations: 1:100 - 1:400 for Tissue Staining; 1:250 - 1:1,000 for Immuno Blotting; 1:1,000 - 1:4,000 for ELISA/FIA
