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Anti-human IgG (whole molecule) rabbit IgG fraction, peroxidase-conjugated , 2 mL
Key Features and Details
Lyophilized horseradish peroxidase (HRP)-conjugated rabbit IgG fraction to human IgG (whole molecule) and buffer salts.
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PRODUCT DESCRIPTION
Product is the lyophilized powder of horseradish peroxidase (HRP)-conjugated rabbit IgG fraction to human IgG (whole molecule) and buffer salts.
Application Notes
This product is suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Immunohistochemistry | Immunoblotting |ELISA
SPECIFICATIONS
SKU: 0855221
Base Catalog Number: 55221
Antibody Type
: Polyclonal Antibody
Concentration
: Total Protein: 6.4 mg/mL Titer: 1:170;000
Conjugate
: HRP
Formulation Details
: Lyophilized
Host
: Rabbit
Preparation Method
: Antibody and highly purified HRP (Rz>3.0) are conjugated under defined conditions to obtain optimally labeled product. Conjugated protein is purified by salt fractionation. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.01% thimerosal, adjusted to standard titer, filtered through a 0.22 µm filter, vialed and lyophilized.
Purity
: Purified
Solubility
: Reconstitution: Reconstitute product with 2.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Do not add sodium azide. Avoid vigorous shaking of the reconstituted material.
Species Reactivity
: Human (Whole Molecule)
Typical Working Concentration
: DILUTION RECCOMENDATIONS: 1:500 - 1:4,000 for Tissue Staining 1:1,000 - 1:8,000 for Immuno Blotting 1:2,000 - 1:16,000 for ELISA/FIA
