TB Green Premix Ex Taq II (Tli RNaseH Plus) is a TB Green Master Mix that effectively suppresses amplification of primer dimers and off-target products, and is therefore recommended for real-time PCR (qPCR) when high specificity is critical. Use this product when primer design has been optimized, but when an increase in specificity of the qPCR assay is still needed. TB Green Premix Ex Taq II (Tli RNase H Plus) includes TB Green, a reagent designed for intercalator-based qPCR.