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B12 Assay Medium (Lactobacillus leichmannii Culture)
B12 Assay Medium (Lactobacillus leichmannii Culture) is used for determining Vitamin B12 concentration by the microbiological assay technique.
Composition*
Ingredients
Vitamin Assay Casamino Acids
Dextrose
Asparagine
Sodium Acetate
Ascorbic Acid
L-Cystine
DL-Tryptophan
Adenine Sulphate
Guanine Hydrochloride
Uracil
Xanthine
Riboflavin
Riboflavin
Biotin
Niacin
p-Aminobenzoic Acid
Calcium Pantothenate
Pyridoxine Hydrochloride
Pyridoxal Hydrochloride
Pyridoxamine Hydrochloride
Folic Acid
Monopotassium Phosphate
Dipotassium Phosphate
Magnesium Sulphate
Sodium Chloride
Ferrous Sulphate
Manganese Sulphate
Manganese Sulphate
g/L
15.0
40.0
0.2
20.0
4.0
0.4
0.4
0.02
0.02
0.02
0.02
0.001
0.001
0.00001
0.002
0.002
0.001
0.004
0.004
0.0008
0.0002
1.0
1.0
0.4
0.02
0.02
0.02
2.0
Application
Pharmaceuticals
Vitamin Assay Media
fdef
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*Adjusted to suit performance parameters
Final pH (at 25°C) 6.0 ± 0.1
g/L: Suspend 85.00 g of the powder in 1000 mL of distilled water.
