10X Trypsin HBSS w/o calcium and magnesium, 100 mL

Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture. Application Notes Trypsin-EDTA solutions are used to release adherent cells from culture surfaces. They are generally composed of variable concentrations of natural porcine pancreas-derived trypsin and EDTA. Trypsin 1X solutions can range from 0.025% to 0.5%. The reasons for the range of concentrations are differences in required trypsin activity or potency; differences in incubation times; and cell line susceptibility. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture. Various formulations should be tested to determine the best product for a specific application. Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. Key Applications Cell Counting | Cell Cell Viability | Cryopreservation | Cell dissociation

Formulation Details : Liquid | Potassium Chloride [KCl]: 400.0000 mg/L; Potassium Phosphate Monobasic [KH₂PO₄]: 60.0000 mg/L; Sodium Bicarbonate [NaHCO₃]: 350.0000 mg/L; Sodium Chloride [NaCl]: 8000.0000 mg/L; Sodium Phosphate Dibasic [Na₂HPO₄]: 47.5000 mg/L; Dextrose: 1000.0000 mg/L; Phenol Red Sodium Salt: 17.0000 mg/L; Trypsin 1:250: 2500.0000 mg/L