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1X Trypsin-EDTA solution, 100 mL
Key Features and Details
0.25% Trypsin (1:250) and 1mM EDTA in modified HBSS w/o Ca++ and Mg++, 100 mL
SYNONYMS: Trypsin (1X), Trypsin-EDTA solution
CAS: # 9002-07-7 (trypsin)
MOLECULAR WEIGHT: Approximately 23;000 (trypsin)
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PRODUCT DESCRIPTION
SPECIFICATIONS
SKU: 091689649
Base Catalog Number: 1689649
Alternate Names: Trypsin (1X), Trypsin-EDTA solution
CAS
: # 9002-07-7 (trypsin)
Concentration
: 0.25% (w/v) solution | 0.25% Trypsin (1:250) and 1mM EDTA In Modified HBSS Without Ca++ and Mg++
Format
: Solution
Formulation Details
: Liquid | 0.25% Trypsin (1:250) and 1mM EDTA In Modified Hbss Without Ca++ and Mg++
Media Type
: Mammalian Media
Molecular Weight
: Approximately 23;000 (trypsin)
pH
: 7.0 - 7.3
Source
: Porcine (for trypsin)
Specific Activity
: 1 TAME unit = 19.2 USP or NF units = 57.5 BAEE Units
Specificity
: Specificity: The protease activity of trypsin is highly specific toward positively charged side chains with lysine and arginine. Forms complexes with a2-macroglobulin. Can be used in the isolation of intact; detergent-free phycobilisomes and in the hydrolysis/condensation of carboxylic ester bonds.
Sterility
: Sterile
Unit Definition
: One BAEE unit will produce a ?A253 of 0.001 per minute at pH 7.6 at 25 °C using BAEE as substrate. Reaction volume = 3.2 mL (1 cm light path). One TAME unit hydrolyzes 1 µmole of p-toluene-sulfonyl-L-arginine methyl ester (TAME) per minute at 25 °C, pH 8.2, in the presence of 0.001 M calcium ion. One USP trypsin unit is the activity causing a change in absorbance of 0.003 per minute under the conditions specified.
