5-Bromo-4-chloro-3-indolyl-ß-D-galactopyranoside

5-Bromo-4-Chloro-3-Indolyl-?-D-Galactopyranoside Application Notes 5-Bromo-4-Chloro-3-Indolyl-ß-D-Galactopyranoside is used as indigogenic substrate for ß-galactosidase, for detection of ß-galactosidase-positive clones, and the identification of lac and bacterial colonies or phage plaques. It is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype. X-Gal is cleaved by ß-galactosidase to yield an insoluble blue precipitate. X-Gal is particularly useful in molecular biology applications to detect the activity of ß-galactosidase which is frequently used as a reporter gene. In cloning, X-Gal is used to detect insertion of foreign DNA into the lacZ region of plasmid DNA using a-complementation which is based on vectors such as the pUC and the M13mp series that carry a fragment of the ß-galactosidase gene encoding an a-fragment of ß-galactosidase. Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. Key Applications Indigogenic substrate for ß-galactosidase