5-FOA (5-fluoroorotic acid), 1 g

5-Fluoroorotic Acid monohydrate is a derivative of a pyrimidine precursor and is selectively toxic to yeast cells which synthesize orotidine-5’-phosphate decarboxylase. Application Notes 5-Fluoroorotic Acid monohydrate (FOA) is used as a selective agent in yeast molecular genetics. Curing yeast strains of plasmids, plasmid shuffling, allelic replacement, and two-hybrid screens are methods that can employ the use of 5-FOA. It is also used in the selection of Ura cells in a population of Ura+ cells; and in the selection of orotidine-5’-phosphate decarboxylase (OMPdecase) mutants of Saccharomyces cerevisiae. FOA (0.1 mg/mL) has been used in the positive selection for uracil auxotrophs of the sulfur-dependent thermophilic archaebacterium Sulfolobus acidocaldarius. It is a noncompetitive inhibitor of dihydroorotase. FOA (50% inhibition, 6 nM) was a selective inhibitor of malarial cells of Plasmodium falciparum in vitro and in vivo. FOA showed anti-tumor activity against transplanted tumors in rats and mice; a bacteriostatic effect in vitro against various microorganisms, particularly gram-negative bacteria; antimycotic activity against various types of mold. Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. Key Applications Cell culture |Proteomics |Purification and Detection |Protein Analysis |Yeast Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Gene Function |Gene Regulation |Automated Bacterial Growth Media |S. cerevisiae Culture

Typical Working Concentration : 1 mg/mL has been used as a selective agent in yeast molecular genetics