No products in the cart.
Anti-cat IgG (whole molecule) goat IgG fraction, fluorescein-conjugated, 2 mL
Key Features and Details
Lyophilized fluorescein-5-isothiocyanate (FITC “Isomer I”)-conjugated goat IgG fraction to cat IgG (whole molecule) and buffer salts
MDL No: MFCD00162212
You need to register an account with us
to be able to place an order, Click to Register.
PRODUCT DESCRIPTION
Product is the lyophilized powder of fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat IgG fraction to cat IgG (whole molecule) and buffer salts
Application Notes
Fluorescein-Conjugated Goat IgG Fraction To Cat IgG (Whole Molecule) is suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Immunoassays | Immunohistochemistry (Paraffin; Acetone-Fixed; 4% PFA Frozen) | Immunofluorescence assays (IFA) | Flow Cytometry (FACS)
SPECIFICATIONS
SKU: 0855291
Base Catalog Number: 55291
Antibody Type
: Polyclonal Antibody
Concentration
: Total Protein: 16.9 mg/mL; Antibody Protein: 6 mg/mL
Conjugate
: FITC
Formulation Details
: Lyophilized
Host
: Goat
Immunogen
: Cat IgG
Isotype
: IgG
MDL No
: MFCD00162212
PPE
: Eyeshields, Gloves, half-mask respirator, multi-purpose combination respirator cartridge
Preparation Method
: Antibody and FITC are conjugated under defined conditions to obtain the specified molar ratio (3-6 mol/mol). Conjugated protein is purified by gel filtration. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 µm filter, vialed, and lyophilized.
Purity
: Purified
Solubility
: lity
Soluble in water
Species Reactivity
: Cat (Whole Molecule)
Specificity
: This product shows reactivity to cat IgG; cross-reactivity to other species may exist. Because the product is directed against whole IgG; some antibodies are expected to bind light chain sites common to all immunoglobulins.
Typical Working Concentration
: Anti-Cat IgG (whole molecule)-FITC antibody may be used for immunofluorescence at a working antibody dilution of 1:32. The antibody was used in indirect fluorescent antibody test (IFAT) to detect Toxoplasma specific antibodies in cat at a dilution of 1:100. Anti-cat IgG (whole molecle)-FITC antibody can be used in direct (1:32) and indirect immunofluorescence (dilution 1:64)
