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Anti-dog IgG (whole molecule) goat IgG fraction, fluorescein-conjugated, 2 mL
Key Features and Details
Lyophilized fluorescein-5-isothiocyanate (FITC “Isomer I”)-conjugated goat IgG fraction to dog IgG (whole molecule) and buffer salts.
MDL No: MFCD00221535
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PRODUCT DESCRIPTION
Product is the lyophilized powder of fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat IgG fraction to dog IgG (whole molecule) and buffer salts.
Application Notes
Fluorescein-Conjugated Goat IgG Fraction to Dog IgG (Whole Molecule) is used as a reagent in immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections. It is also suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Immunoassays | Immunohistochemistry (Paraffin; Acetone-Fixed; 4% PFA Frozen) | Immunofluorescence assays (IFA) | Flow Cytometry (FACS)
SPECIFICATIONS
SKU: 0855325
Base Catalog Number: 55325
Antibody Type
: Polyclonal Antibody
Concentration
: Total Protein: 21.9 mg/ml F/P Ratio: 5.95 mol/mol Antibody Protein: 8 mg/m
Conjugate
: FITC
Formulation Details
: Lyophilized
Host
: Goat
Immunogen
: Dog IgG
MDL No
: MFCD00221535
PPE
: Eyeshields, Gloves, half-mask respirator, multi-purpose combination respirator cartridge
Preparation Method
: Antibody and FITC are conjugated under defined conditions to obtain the specified molar ratio (3-6 mol/mol). Conjugated protein is purified by gel filtration. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 µm filter, vialed, and lyophilized.
Purity
: Purified
Solubility
: lity
Soluble in water
Species Reactivity
: Dog (Whole Molecule)
Specificity
: This product shows reactivity to dog IgG; cross-reactivity to other species may exist. Because the product is directed against whole IgG; some antibodies are expected to bind light chain sites common to all immunoglobulins.
Typical Working Concentration
: Suggested working dilution: Direct immunofluorescence: 1:32
