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Anti-human IgA (alpha chain) goat F(Ab')2 fragment, fluorescein-conjugated, 2 mL
Key Features and Details
Lyophilized fluorescein-5-isothiocyanate (FITC “Isomer I”)-conjugated goat F(ab’)2 fragment to human IgA (a chain) and buffer salts.
MDL No: MFCD00162415
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PRODUCT DESCRIPTION
Fluorescein-conjugated Goat F(ab')2 fragment to Human IgA is the lyophilized powder of fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat F(ab')2 fragment to human IgA (a chain) and buffer salts.
Application Notes
Fluorescein-conjugated goat F(ab')2 fragment to human IgA (a chain) is used as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. Note: Affinity purified antibodies and their F(ab')2 fragments are recommended to avoid non-specific binding from the inherent antibodies of host animals. A dilution of 1:100 was used for immunohistochemistry of cryosections of human sural nerves.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Immunoassays | Immunohistochemistry (Paraffin; Acetone-Fixed; 4% PFA Frozen) | Immunofluorescence assays (IFA) | Flow Cytometry (FACS)
SPECIFICATIONS
SKU: 0855178
Base Catalog Number: 55178
Antibody Type
: Polyclonal Antibody
Concentration
: Total Protein: 13.8 mg/mL; Antibody Protein: 6 mg/mL
Conjugate
: FITC
Formulation Details
: Lyophilized
Host
: Goat
Immunogen
: Purified human IgA
MDL No
: MFCD00162415
Preparation Method
: Antibody and FITC are conjugated under defined conditions to obtain the specified molar ratio (3-6 mol/mol). Conjugated protein is purified by gel filtration. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 µm filter, vialed, and lyophilized.
Purity
: Purified
Solubility
: Reconstitution: Reconstitute product with 2.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material.
Species Reactivity
: Human (Alpha Chain)
Specificity
: This product shows reactivity to human IgA; cross-reactivity to other species may exist. Antibody activity to IgG; IgM; or non-immunoglobulin serum proteins is not present.
Typical Working Concentration
: Dilution Recommendations: 1:100 - 1:400 for Tissue Staining; 1:250 - 1:1,000 for Immuno Blotting; 1:1,000 - 1:4,000 for ELISA/FIA
