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Anti-human IgG (whole molecule) affinity-purified goat antibody, 2 mg
Key Features and Details
Affinity purified Goat antibody to human IgG (whole molecule) and buffer salts.
MDL No: MFCD00162452
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PRODUCT DESCRIPTION
Goat affinity purified antibody to human IgG (Whole Molecule) is goat affinity purified antibody to human IgG (whole molecule) and buffer salts.
Application Notes
IgG, Whole Molecule Polyclonal Antibody is used in immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections. May be used in quantitative precipitin assay. This product is suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. It is also used for conjugation with enzymes, radiolabels, fluorochromes or other markers, for attachment to solid supports for use as an immunoadsorbant, as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining. These affinity purified antibodies are recommended to avoid non-specific binding from the inherent antibodies of host animals. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Immunoassays | Immunohistochemistry (Paraffin; Acetone-Fixed) | Immunofluorescence assays (IFA)
SPECIFICATIONS
SKU: 0855070
Base Catalog Number: 55070
Antibody Type
: Polyclonal Antibody
Concentration
: Total Protein: 1.08 mg/mL; Active Antibody: 1.0 mg/mL
Conjugate
: Unconjugated
Formulation Details
: Liquid
Host
: Goat
Immunogen
: Human IgG
MDL No
: MFCD00162452
Preparation Method
: The affinity purified antibody is prepared from the specific goat IgG fraction by solid phase absorption. The resulting affinity purified antibody is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 µm filter, and vialed. No perservative has been added.
Purity
: Affinity Purified
Solubility
: Soluble in Water
Species Reactivity
: Human (Whole Molecule)
Specificity
: Product shows reactivity to human IgG; cross-reactivity to other species may exist. Because the product is directed against whole IgG; some antibodies are expected to bind light chain sites common to all immunoglobulins.
Typical Working Concentration
: Quantitative precipitin assay: 3.0 mg/mL, 1:50 - 1:200 for Tissue Staining, 1:100 - 1:500 for Immunoblotting, 1:400 - 2,000 for Elisa
