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Anti-human IgG (whole molecule) affinity-purified goat F(Ab')2 fragment, fluorescein-conjugated, 1 mg
Key Features and Details
Lyophylized fluorescein-5-isothiocyanate (FITC “Isomer I”)-conjugated goat affinity purified F(ab’)2 fragments to human IgG (whole molecule) and buffer salts.
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PRODUCT DESCRIPTION
Fluorescein-Conjugated Goat Affinity Purified F(ab')2 Fragment to Human IgG (Whole Molecule) is fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat affinity purified F(ab')2 fragments to human IgG (whole molecule) and buffer salts.
Application Notes
Fluorescein-Conjugated Goat Affinity Purified F(ab')2 Fragment to Human IgG (Whole Molecule) is suitable for use in direct immunofluorescence, as well as for ANA (Anti-Nuclear Antibody)-indirect immunofluorescence. The antibody may also be used in FACS-based assays. It is also suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections, and blot immunostaining. Use of affinity purified antibodies is recommended to avoid non-specific binding from the inherent antibodies of host animals; these F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Immunoassays | Immunohistochemistry (Paraffin; Acetone-Fixed; 4% PFA Frozen) | Immunofluorescence assays (IFA) | Flow Cytometry (FACS)
SPECIFICATIONS
SKU: 0855201
Base Catalog Number: 55201
Antibody Type
: Polyclonal Antibody
Concentration
: Total Protein: 1.07 mg/mL; Active Antibody: 1.0 mg/mL
Conjugate
: FITC
Formulation Details
: Liquid
Host
: Goat
Immunogen
: Pooled normal human serum
PPE
: Eyeshields, Gloves, half-mask respirator, multi-purpose combination respirator cartridge
Preparation Method
: Antibody and FITC are conjugated under defined conditions to obtain the specified molar ratio (3-6 mol/mol). Conjugated protein is purified by gel filtration. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide and 10% glycerol (v/v), adjusted to standard titer, filtered through a 0.22 µm filter, and vialed.
Purity
: Affinity Purified
Solubility
: lity
Soluble in Water
Species Reactivity
: Human (Whole Molecule)
Specificity
: This product shows reactivity to human IgG; cross-reactivity to other species may exist. Because the product is directed against whole IgG; some antibodies are expected to bind light chain sites common to all immunoglobulins.
Typical Working Concentration
: ANA (Anti-Nuclear Antibody)-indirect immunofluorescence: 1:32; Direct immunofluorescence: 1:16. Dilution recommended: 1:100 - 1:400 for Tissue Staining; 1:250 - 1:1,000 for Immuno Blotting; 1:1,000 - 1:4,000 for ELISA/FIA.
