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Anti-human IgG (whole molecule) goat IgG fraction, peroxidase-conjugated , 2 mL
Key Features and Details
Lyophilized horseradish peroxidase (HRP)-conjugated goat IgG fraction to human IgG (whole molecule) and buffer salts.
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PRODUCT DESCRIPTION
Peroxidase-Conjugated Goat IgG Fraction to Human IgG (Whole Molecule) is the lyophilized powder of horseradish peroxidase (HRP)-conjugated goat IgG fraction to human IgG (whole molecule) and buffer salts.
Application Notes
Peroxidase-Conjugated Goat IgG Fraction to Human IgG (Whole Molecule) has been used in dot blot. It is suitable for use as a reagent in enzyme immunoassays (EIA), cell and immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections, cell and tissue labeling (for electron microscopy), and blot immunostaining. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies or their fragments are recommended to avoid non-specific binding from inherent antibodies of host animals.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Immunohistochemistry | Immunoblotting |ELISA
SPECIFICATIONS
SKU: 0855220
Base Catalog Number: 55220
Antibody Type
: Polyclonal Antibody
Concentration
: Total Protein: 5.7 mg/mL; Titer: 1:197;000
Conjugate
: HRP
Formulation Details
: Lyophilized
Host
: Goat
Preparation Method
: Antibody and highly purified HRP (Rz>3.0) are conjugated under defined conditions to obtain optimally labeled product. Conjugated protein is purified by salt fractionation. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.01% thimerosal, adjusted to standard titer, filtered through a 0.22 µm filter, vialed and lyophilized.
Purity
: Purified
Solubility
: Reconstitution: Reconstitute product with 2.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Do not add sodium azide. Avoid vigorous shaking of the reconstituted material.
Species Reactivity
: Human (Whole Molecule)
Specificity
: It shows reactivity to human IgG; cross-reactivity to other species may exist. Because the product is directed against whole IgG; some antibodies are expected to bind light chain sites common to all immunoglobulins.
Typical Working Concentration
: 1:50 - 1:200 for Tissue Staining 1:100 - 1:500 for Immunoblotting 1:400 - 2,000 for Elisa
