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Anti-human IgM (5Fc µ) goat IgG fraction, 5 mL
Key Features and Details
Lyophilized goat IgG fraction to human IgM (5Fc µ) and buffer salts.
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PRODUCT DESCRIPTION
Product is the lyophilized powder of goat IgG fraction to human IgM (5Fc µ) and buffer salts.
Application Notes
Goat IgG fraction to Human IgM is use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. It is suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant.It is also suitable for use as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies are recommended to avoid non-specific binding from the inherent antibodies of host animals).
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Immunoassays | Precipitating antibody |Immunoprecipitation
SPECIFICATIONS
SKU: 0855017
Base Catalog Number: 55017
Antibody Type
: Polyclonal Antibody
Concentration
: Total Protein: 7.8 mg/mL; Antibody Protein: 3.5 mg/mL
Conjugate
: Unconjugated
Formulation Details
: Powder
Host
: Goat
Preparation Method
: The IgG fraction is prepared from the specific goat antiserum by delipidation, ammonium sulfate fractionation and anion exchange chromatography. The resulting IgG fraction is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 µm filter, vialed and lyophilized.
Purity
: IgG Fraction
Solubility
: Reconstitution: Reconstitute product with 5.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material.
Species Reactivity
: Human, IgM (5Fc µ)
Specificity
: Product shows reactivity to human IgM; cross-reactivity to other species may exist. Antibody activity to human IgG; IgA or non-immunoglobulin serum proteins is not present.
