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Anti-rabbit IgG (whole molecule) goat IgG fraction, peroxidase-conjugated, 2 mL
Key Features and Details
Lyophilized horseradish peroxidase (HRP)-conjugated goat IgG fraction to rabbit IgG (whole molecule) and buffer salts. IgG fraction of antiserum is essentially free of other goat serum proteins.
MDL No: MFCD00162487
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PRODUCT DESCRIPTION
Peroxidase-conjugated goat igg fraction to rabbit IgG (whole molecule) is the lyophilized powder of horseradish peroxidase (HRP)-conjugated goat IgG fraction to rabbit IgG (whole molecule) and buffer salts. Anti-Human IgG is developed in goat using IgG from pooled normal human serum as the immunogen. Whole antiserum is fractionated and then further purified by ion exchange chromatography to provide the IgG fraction of antiserum. This fraction is essentially free of other goat serum proteins.
Application Notes
Peroxidase-conjugated goat IgG fraction to rabbit IgG (whole molecule) product is suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies or their fragments are recommended to avoid non-specific binding from inherent antibodies of host animals.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Immunohistochemistry | Immunoblotting |ELISA
SPECIFICATIONS
SKU: 0855676
Base Catalog Number: 55676
Antibody Type
: Polyclonal Antibody
Concentration
: Total Protein: 6.9 mg/mL Titer: 1:180;000
Conjugate
: HRP
Formulation Details
: Lyophilized
Host
: Goat
MDL No
: MFCD00162487
PPE
: Eyeshields, Gloves, half-mask respirator , multi-purpose combination respirator cartridge
Preparation Method
: Antibody and highly purified HRP (Rz>3.0) are conjugated under defined conditions to obtain optimally labeled product. Conjugated protein is purified by salt fractionation. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 µm filter, vialed and lyophilized.
Purity
: Purified
Solubility
: Soluble in water
Species Reactivity
: Rabbit (Whole Molecule)
Specificity
: It shows reactivity to rabbit IgG; cross-reactivity to other species may exist. Because the product is directed against whole IgG; some antibodies are expected to bind light chain sites common to all immunoglobulins. Antibody activity to non-immunoglobulin serum proteins is not present.
Typical Working Concentration
: Anti-Human IgG (whole molecule)-HRP antibody is suitable for use in direct immunofluorescence (1:16), as well as for ANA-indirect immunofluorescence at 1:32 or 1:641 dilutions. The antibody may also be used in FACS-based assays (1:200).
