Poly-D-lysine hydrobromide, Molecular Weight, 70,000-150,000

Poly-D-Lysine Hydrobromide Application Notes It is typically used as a coating substrate for culture dishes, slides, etc. It enhances electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. Both the D- and L- form of the poly-lysine can be used as a coating substrate since poly-lysine is a nonspecific attachment factor for cells; however, certain cells can digest poly-lysine. In this case, poly-D-lysine should be used as the attachment factor so that the cells are not disrupted by excessive uptake of L-lysine.Other uses for poly-lysine have been reported as well:• Conjugation to methotrexate for increased drug transport.• Microencapsulation of islets.• Use in simple reproducible procedure for chromosomal preparations from a variety of tissues.• Immobilation of living cells in biocompatible semipermeable microcapsules.• In the preparation of polycationic beads.• Conjugation to albumin and horseradish peroxidase to enhance cellular uptake. Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. Key Applications Cell adhesion