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YES, powder
Key Features and Details
YES Agar is a nutritionally rich medium for the growth of S. pombe containing dextrose, yeast extract and agar.
SYNONYMS: Edinburgh's Minimal Medium |S.pombe growth medium
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PRODUCT DESCRIPTION
YES is a nutritionally rich medium for the growth of S. pombe, containing dextrose, and yeast extract. Dextrose is the energy source. Yeast extract provides carbon, nitrogen, minerals, vitamins, trace ingredients and other essential growth nutrients which stimulate the growth of yeasts.
Application Notes
EMM (Edinburgh Minimal Medium) is used for the growth of fission yeast S. Pombe.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Cell culture |Proteomics |Purification and Detection |Protein Analysis |Yeast Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Gene Function |Gene Regulation |Automated Bacterial Growth Media |S. pombe Culture
SPECIFICATIONS
SKU: 114101522-CF
Base Catalog Number: 4101522
Alternate Names: Edinburgh's Minimal Medium |S.pombe growth medium
Format
: Powder
Formulation Details
: Contents Per Liter: 5 g Yeast Extract-Y; 30 g Dextrose; 1X Supplements (50 mg each ADE; HIS; LEU; LYS; URA). Small quantities of biological buffers have been added for proper pH adjustment.
Grade
: Molecular Biology Certified
Media Type
: YES
pH
: pH Range: 5.5 - 6.5 at 25 °C
PPE
: Personal Protective Equipment Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: PVC or other plastic material gloves Skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice
Preparation Method
: Media Preparation: Reagents are tested and chosen for their ability to promote optimum growth of S. pombe when combined in YES Broth formulations. Chosen reagents are combined in the proper proportions according to the YES formulation and thoroughly blended for a uniform distribution. A sample of the blended formulation is used to prepare liquid medium. The combinations are mixed to dissolve components and then autoclaved at 121 °C for 15 minutes.
Solubility
: Soluble in water (35 g/L).
