NucleoBond PC EF Kits use anion exchange and gravity flow for purification of endotoxin-free plasmid DNA. Endotoxin removal is achieved in a unique washing step—no extra incubations are necessary. Lysates are cleared by simple filtration using the included NucleoBond Folded Filters; no centrifugation is required. NucleoBond PC 500 EF is used to purify 100–500 µg of endotoxin-free plasmid DNA.