One Step PrimeScript III RT-qPCR Mix is a 2X master mix for one-step real-time, probe-based RT-qPCR (using the 5′ nuclease method). It contains a novel heat- and inhibitor-resistant reverse transcriptase, PrimeScript III RTase, in combination with an efficient, specific hot-start PCR enzyme, TaKaRa Taq HS, and an optimized reaction buffer. A quick and simple protocol allows the reverse transcription and qPCR reactions to be performed in the same tube, reducing the risk of contamination.