Real-time RT-PCR is made easy with the One Step PrimeScript RT-PCR Kit (Perfect Real Time), a one-step real-time RT-PCR kit designed for real-time RT-PCR (qPCR) via probe-based qPCR assays such as 5' nuclease assays1. In this 5' nuclease assay real-time PCR (qPCR) kit, one-step real-time RT-PCR can be performed in a single tube, simplifying amplification and minimizing the risk of contamination. Amplified products are monitored in real time, so there is no need to verify them through electrophoresis following PCR. The sensitivity provided by the enzymes in this kit enables detection of very small amounts of RNA, including RNA derived from viral, rare, or precious samples.